Transformation of Salmonella typhimurium by Plasmid Deoxyribonucleic Acid
نویسندگان
چکیده
منابع مشابه
Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.
A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described. Transformed bacteria acquire a circular deoxyribonucleic acid species having the genetic and molecular characteristics of the transforming plasmid.
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The genetic transformation of Escherichia coli K-12 with chromosomal deoxyribonucleic acid purified from Salmonella typhi is described.
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The rate of thymidine incorporation into cells of Salmonella typhimurium growing in different media has been measured. In glucose-minimal medium, deoxyribonucleic acid (DNA) replication occurs during the first two-thirds of the division cycle; the final one-third of the division cycle was devoid of DNA replication. The measured doubling time of S. typhimurium in this medium is approximately 48 ...
متن کاملMolecular nature of R-factor deoxyribonucleic acid isolated from Salmonella typhimurium minicells.
In earlier reports it was shown that a variety of extrachromosomal elements harbored in the Escherichia coli minicell producer segregate into the minicells. We show in this report that the fi(+) R factor R-100-1 (derepressed derivative of R-100) similarly segregates into minicells produced by a Salmonella typhimurium strain. Four distinct classes of covalently closed circular deoxyribonucleic a...
متن کاملThe recombination of bromouracil-containing deoxyribonucleic acid in Salmonella typhimurium transduction.
Salmonella typhimurium tolerated the replacement of about 70 % of the thymine in the DNA by 5-bromouracil (BU). In phage PLT-22 transduction, the presence of BU in the donor DNA had no effect on the linkage relationship of two contransduced markers and did not conclusively alter the ratio of complete transductants to abortive transductants. The yield of transductants per plaque-forming unit was...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1974
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.119.3.1072-1074.1974